GC-MS Based Profiling of Anti-Analgesic Components in Creeping Launaea [Launaea Procumbens (Roxb.) Ramayya & Rajagopal]: In Silico and In Vivo Approaches

Abstract

The pharmacists use Launaea procumbens, because it is a good potential 
for Galenical's. The current investigation was focused on 
pharmacognostic characterization of the various parts of L. procumbens 
(Roxb.) Ramayya Rajagopal, family Asteraceae. The herb of L. 
procumbens frequently employed to relieve fever, pain and 
inflammation. The goal of this work is to develop standardization 
parameters of L. procumbens in in vivo, in vitro and in silico analysis. 
The ethanolic extract of L. procumbens has an analgesic, anti
inflammatory, anti-microbial and molecular docking consequence. In 
the in vitro studies, the egg albumin protein denaturation assay and 
antimicrobial activity was performed to check the inhibition of protein 
and zone of inhibition of microbial growth. The three triterpene 
compounds; lupeol, alpha amyrin and beta amyrin were identified from 
the L. procumbens ethanolic extract (LPEE) in gas chromatography mass spectrometry (GCMS) analysis. These compounds were used as a ligands, interact them with proteins cyclooxygenase 2 (COX2) and interleukin 17 
(IL-17), the indomethacin were used as a reference drug (ligand).